Table 2 N -glycan microheterogeneity of human GGT1 expressed in Pichia pastoris as determined by LC-MS/MS (see Figure 4 )
hGGT1 | ||||
|---|---|---|---|---|
N -glycosylation | Theoretical | Mass | MS/MS | |
Site | N -glycan | Mass | Measured | Support |
[M + 3H] + 3 | ||||
N120 | Man8GlcNAc2 | 1131.1307 | 1131.1153 | ✓ |
Man9GlcNAc2 | 1185.1483 | 1185.1520 | ✓ | |
Man10GlcNAc2 | 1239.1659 | 1239.1576 | ✓ | |
Man11GlcNAc2 | 1293.1835 | 1293.1609 | ✓ | |
Man12GlcNAc2 | 1347.2011 | 1347.1885 | ✓ | |
Man13GlcNAc2 | 1401.2187 | 1401.2055 | ✓ | |
[M + 3H] + 3 | ||||
N344 | Man7GlcNAc2 | 985.7410 | 985.7221 | ✓ |
Man8GlcNAc2 | 1039.7586 | 1039.7490 | ✓ | |
Man9GlcNAc2 | 1093.7762 | 1093.7745 | ✓ | |
Man10GlcNAc2 | 1147.7938 | 1147.7869 | ✓ | |
Man11GlcNAc2 | 1201.8114 | 1201.8104 | ✓ | |
Man12GlcNAc2 | 1255.829 | 1255.8137 | ✓ | |
[M + 3H] + 3 | ||||
N511 | Man8GlcNAc2 | 1112.8331 | 1112.8189 | |
Man9GlcNAc2 | 1166.8507 | 1166.8450 | ✓ | |
Man10GlcNAc2 | 1220.8683 | 1220.8614 | ✓ | |
Man11GlcNAc2 | 1274.8859 | 1274.8865 | ✓ | |
Man12GlcNAc2 | 1328.9035 | 1328.8865 | ✓ | |
Man13GlcnNAc2 | 1382.9211 | 1832.9140 | ✓ | |