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Figure 8 | BMC Biotechnology

Figure 8

From: Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology

Figure 8

hGGT1 lectin blotting confirms differential ALSA binding affinities. Membrane extracts from normal human kidney and liver tissue or Pichia pastoris-expressed hGGT1 were activity-normalized and subjected to immunoprecipitation with a polyclonal anti-hGGT1 large subunit antibody. Equal volumes from each immunoprecipitation eluate were resolved by SDS-PAGE and affinity blotted with anti-hGGT1 (hGGT1, top panel) or the biotinylated lectins, microvirin (MVN, second panel), Phaseolus vulgaris Erythroagglutinin (Pha-E, third panel), and Datura stramonium lectin (DSL, bottom panel). Expanded views of the immunoblots shown here along with the relevant molecular weight markers are included in Additional file 1.

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