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Figure 3 | BMC Biotechnology

Figure 3

From: Generation in yeast of recombinant virus-like particles of porcine circovirus type 2 capsid protein and their use for a serologic assay and development of monoclonal antibodies

Figure 3

Titration curves of swine serum samples. Either positive or negative for PCV2 antibodies swine serum samples were titrated by the newly developed indirect IgG PCV2 Cap VLP-based ELISA using recombinant PCV2 Cap VLPs at a concentration of 0.5 μg/ml (solid lines) and control antigen (HaPyV-VP1) at a concentration of 2 μg/ml (dotted line). Sera were tested in serial duplicate twofold dilutions ranging from 1:100 to 1:3200. The results are expressed as the OD450 and correspond to the average of the values obtained in at least two different analyses.

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