Figure 1

Analysis of recombinant PCV2 Cap protein-derived VLPs produced in yeast. (A) Coomassie blue-stained SDS-PAGE; (B) Western blot with pig serum. (A,B): In lanes: 1 - whole crude lysate of yeast transformed with pFX7-PCV2Cap-521 plasmid, 2 - the soluble fraction of the lysate of yeast transformed with pFX7-PCV2Cap-521 plasmid; 3 - whole crude lysate of yeast transformed with pFX7- pFX7-PCV2Cap-113 plasmid; 4 - the soluble fraction of the lysate of yeast transformed with pFX7-PCV2Cap-113 plasmid; 5 - whole crude lysate of yeast transformed with pFX7-PCV2Cap-622 plasmid; 6 - the soluble fraction of the lysate of yeast transformed with pFX7-PCV2Cap-622 plasmid; 7 - whole crude lysate of yeast transformed with pFX7-PCV2Cap-622S plasmid; 8 - the soluble fraction of the lysate of yeast transformed with pFX7-PCV2Cap-622S plasmid; 9 - whole crude lysate of yeast transformed with pFX7 plasmid, M- pre-stained protein weight marker (Thermo Fisher Scientific Baltics); 10 - Cap521 protein VLPs purified by density gradient centrifugation; 11 - Cap113 protein VLPs purified by density gradient centrifugation; 12 – Cap622 protein VLPs purified by density gradient centrifugation; 13 - Cap622S protein VLPs (Cap622 protein VLPs generated using synthetic codon-optimized gene) purified by density gradient centrifugation. (C): Electron microscopy pictures of VLPs formed by PCV2-Cap521, protein PCV2-Cap113, protein PCV2-Cap622 and protein PCV2-Cap622S protein stained with 2% aqueous uranyl acetate solution and examined by Morgagni 268 electron microscope.