Table 1 Formation of mAb dimer, oligomer, fragment and large aggregates using the different induction methods
From: Direct analysis of mAb aggregates in mammalian cell culture supernatant
mAb1 | mAb2 | ||||||
|---|---|---|---|---|---|---|---|
Dimer (%) | Oligomer (%) | Zave (nm) | Dimer (%) | Oligomer (%) | Fragment (%) | Zave (nm) | |
pH | |||||||
3 | 1.8 | - | 34.2 ± 1.8 | 1.7 | 1.8 | - | 39.6 ± 2.4 |
4 | 1.9 | - | 32.5 ± 3.3 | 2.1 | 0.9 | - | 49.5 ± 3.5 |
5 | 2.3 | - | 84.3 ± 15.0 | 2.0 | 0.9 | 3.5 | 144.9 ± 35.8 |
6 | 2.6 | - | 85.7 ± 1.9 | 3.5 | 0.6 | 5.7 | 47.9 ± 1.3 |
7 | 3.1 | - | 51.1 ± 5.3 | 4.0 | 0.5 | 4.5 | 45.7 ± 0.8 |
8 | 3.4 | - | n. a. | 6.3 | 0.6 | 6.3 | n. a. |
NaCl | |||||||
Control | 0.6 | - | 33.7 ± 5.3 | 1.1 | - | - | 33.2 ± 0.2 |
500 mM | 1.3 | - | 23.8 ± 0.2 | 3.0 | - | - | 41.8 ± 1.9 |
1 M | 1.7 | - | 24.8 ± 0.6 | 4.2 | - | - | 56.6 ± 8.8 |
1.5 M | 2.0 | - | 24.2 ± 1.6 | 4.6 | - | - | 78 ± 9.2 |
FT | |||||||
Control | 0.6 | - | 19.7 ± 1.1 | 1.4 | - | - | 10.6 ± 1.1 |
1× | 16.0 | 11.3 | 20.7 ± 1.8 | 17.1 | 3.6 | - | 14.4 ± 0.1 |
2× | 20.2 | 16.3 | 26.6 ± 1.9 | 19.3 | 3.9 | - | 12.9 ± 0.5 |
3× | 22.7 | 11.0 | 27.3 ± 2.8 | 21.0 | 6.6 | - | 12.1 ± 0.5 |