Figure 4
From: Direct analysis of mAb aggregates in mammalian cell culture supernatant
Analysis of mAb aggregate formation, DNA content, host cell and medium components in the supernatant of a mAb2-producing CHO cell line. Supernatant was analyzed for mAb aggregate formation directly after inoculation and after 144 h cultivation (A + B). Amount of mAb2 monomer, dimer and oligomers was obtained from biological triplicates ± standard deviation. B is an enlargement of A. DNA content of collected SEC fractions was analyzed using NanoDrop 1000 spectrophotometer (C). For determination of HCPs and culture medium components, SEC fractions were pooled, concentrated and analyzed using SDS-PAGE under reducing conditions with subsequent silver staining (D).