Figure 3
From: Morphology and ploidy level determination of Pteris vittata callus during induction and regeneration
Representative callus on Yang semi-solid medium for six weeks (A and B) and regenerating callus grown on differentiation medium under light culture conditions for four weeks (C) and eight weeks (D). A) Spontaneous sporophytic growth occurred on callus during maintenance on callus induction medium. B) Typical round callus cells present on the callus before sporophytic growth. An extracellular matrix extends from cells. C) Asynchronous differentiation of sporophytes begins with clusters of cells from the callus surface when induced on differentiation medium. Differentiation of sporophytes was asynchronous. D) A crozier of a maturing sporophyte.