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Table 4 Ability of each kit to effectively remove DNA from small tissue samples

From: A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

  Pre-DNase Post-DNase
  no RT RT Difference no RT RT Difference
Plus Mini       
Blood 33.65 31.71 1.94 > 40* 32.23 7.77
Spleen 39.84* 28.62 11.22 39.84* 29.59 10.25
Kidney 38.06* 19.35 18.71 > 40* 23.41 16.59
Embryo > 40* 27.82 12.18 > 40* 29.46 10.54
Larvae > 40* 26.26 13.74 > 40* 27.86 12.14
Plus Universal       
Blood 33.27 32.87 0.40 > 40* 34.07 5.93
Spleen 34.77 28.90 5.87 39.92* 29.14 10.78
Kidney 36.50* 21.54 14.96 > 40* 22.18 17.82
Embryo 39.79* 29.23 10.56 > 40* 31.17 8.83
Larvae > 40* 20.74 19.26 > 40* 28.79 11.21
Maxwell®       
Blood > 40* 34.19 5.81 > 40* 34.84 5.16
Spleen > 40* 29.38 10.62 > 40* 30.25 9.75
Kidney > 40* 20.52 19.48 > 40* 20.67 19.33
Embryo 37.09* 26.41 10.68 > 40* 25.86 14.14
Larvae > 40* 26.46 13.54 > 40* 27.55 12.45
MagMAX ®       
Blood 29.79 28.11 1.68 39.02* 32.65 6.37
Spleen > 40* 29.93 10.07 38.04* 30.36 7.68
Kidney 31.83 21.89 9.94 39.74* 21.51 18.23
Embryo 36.34* 22.57 13.77 > 40* 22.31 17.69
Larvae 33.75 27.54 6.21 > 40* 28.17 11.83
SimplyRNA HT       
Blood > 40* 31.06 8.94 > 40* 31.55 8.45
Spleen > 40* 28.11 11.89 > 40* 27.9 12.10
Kidney > 40* 21.54 18.46 > 40* 21.94 18.06
Embryoa - - - - - -
Larvae > 40* 26.22 13.78 > 40* 26.00 14.00
  1. The threshold cycle (Ct) values of samples subjected to the kit DNase treatment only (pre-DNase) or to an additional DNase treatment, as well as the corresponding "no RT" samples (see Methods for details). *represents no RT samples with Ct values ≥35 and thus, considered gDNA free. indicates samples for which difference between the Ct values of the no RT and RT samples was ≥5, an indicator of sufficient gDNA removal for qPCR gene expression analysis.
  2. aThe quantities of RNA isolated from embryos using the SimplyRNA HT kit were less than the amount necessary for the first-strand synthesis protocol used in this study; therefore, qPCR reactions could not be performed with these samples.