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Table 3 The minimum quantity and quality criteria for RNA used to evaluate the suitability of RNA extracted via each kit for analysis via real-time quantitative (qPCR), microarray or next generation sequencing (NGS) technologies

From: A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

  Total RNA quantity A260:A280ratios RNA integrity number (RIN)
qPCR ≥ 1 μg ≥ 1.8 ≥ 5
Microarray ≥ 5 μg ≥ 1.8 ≥ 7
NGS ≥ 1 μg ≥ 1.8 ≥ 7