Table 3 The minimum quantity and quality criteria for RNA used to evaluate the suitability of RNA extracted via each kit for analysis via real-time quantitative (qPCR), microarray or next generation sequencing (NGS) technologies
Total RNA quantity | A260:A280ratios | RNA integrity number (RIN) | |
|---|---|---|---|
qPCR | ≥ 1 μg | ≥ 1.8 | ≥ 5 |
Microarray | ≥ 5 μg | ≥ 1.8 | ≥ 7 |
NGS | ≥ 1 μg | ≥ 1.8 | ≥ 7 |