The quantity of RNA extracted from fathead minnow tissues using five commercially available RNA extraction kits. The RNA amounts shown in panels on the left (A, C, E, G and I) reflect the total amount of RNA recovered (ng), while the RNA amounts reported in panels on the right (B, D, F, H and J) indicate the amount (ng) of RNA recovered per mass (mg) of tissue. The reported p-values were obtained by one-way ANOVA, except for those denoted by a "W". The "W" indicates that significant differences in variance were detected and that analysis was conducted via a Welch ANOVA. Error bars indicate standard deviation. Different letters above bars indicate significant differences in the measured RNA quantity parameter between samples processed by each of the kits. Percentages reported in each bar are indicative of the coefficient of variance.