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Table 2 Effects of metal ions and chemical reagents on the activity of purified An SHMT a

From: A novel serine hydroxymethyltransferase from Arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design

Reagents

Concentration (mM or %)

Relative activity (%)

control

0

100%

Hg2+

1

- b

Co2+

1

60.03 ± 0.27 c

K+

1

100.44 ± 0.37

Na+

1

100.62 ± 0.30

NH4+

1

95.12 ± 0.58

Sl2+

1

94.44 ± 0.83

Mg2+

1

108.38 ± 0.85

Ca2+

1

91.38 ± 0.11

Ba2+

1

102.58 ± 0.33

Cu2+

1

8.95 ± 0.36

Zn2+

1

2.91 ± 0.11

Mn2+

1

60.81 ± 0.28

Fe2+

1

- b

Pb2+

1

98.90 ± 0.32

CO(NH2)2

1

60.59 ± 0.45

DTT

1

59.29 ± 0.08

CTAB

0.3%

97.05 ± 0.04

EDTA

1%

101.25 ± 0.65

SDS

5%

- b

  1. a: all assays were performed in the standard conditions and the activity measured without additional reagents and ions was taken as 100%. The data are the average of three replicates; b: unmeasured data; c: Relative activity ± the standard deviation.