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Figure 5 | BMC Biotechnology

Figure 5

From: A novel serine hydroxymethyltransferase from Arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design

Figure 5

Effects of temperature and pH on activity. (a) Effect of temperature on the activity of the AnSHMT and the I249L-SHMT. The optimal temperature was determined by measuring the activity at temperatures from 0 to 70°C. The maximal activity was taken as 100%. (b) Effect of pH on the activity of the enzymes. The activity was measured over a pH values ranging from 2.5 to 10.5, and the maximal activity was taken as 100%. (c) Effect of temperature on the stability of the AnSHMT and the I249L-SHMT. At the optimal pH 7.5, the purified enzyme was pre-treated at a different temperature for 1 h. The activity of the enzyme without pre-incubation was defined as 100%. (d) The pH stability of the enzymes was determined by incubating the enzymes at a different pH at 4°C for 24 h. Then assays were conducted in the standard conditions and the enzyme activity without pre-treatment was taken as 100%. Error bars represent the standard deviation. Black circles represent the AnSHMT (), and black squares represent the I249L-SHMT (■).

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