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Table 1 Expression vectors and their fusion tags

From: Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli

Lane Vector Fusion tag BL21 Rosetta 2
20°C PPB 37°C PPB 25°C TBONEX 20°C PPB 37°C PPB 25°C TBONEX
1 pOPINF 6-His- 1 1 1 1 1 1
2 pOPINS3C 6-His-SUMO- 0.7 1.5 2.1 1.3 2.7 1.1
3 pOPINTRX 6-His-Thioredoxin- 1.2 1.7 4.9 1.1 0.7 0.1
4 pOPINMSYB 6-His-MsyB- 0.5 0.8 2.9 1.8 3.6 2.0
5 pOPINJ 6-His-GST- 0.3 1.3 0.4 0.1 0.5 0.1
6 pOPINHALO7 6-His-Halo- 1.5 1.6 1.7 1.6 3.0 2.0
7 pOPINM 6-His-MBP- 0.8 1.4 2.5 0.9 1.1 0.7
8 pOPINTF 6-His-Trigger Factor- 0.5 1.1 1.7 0.3 0.6 0.5
9 pOPINNusA 6-His-NusA- 0.6 1.2 1.1 0.7 0.8 0.1
  1. Empirical scores of soluble fraction yield in small-scale expression relative to 6-His-construct. Highlighted text in bold within the vector name symbolize the fusion tag.