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Table 1 Expression vectors and their fusion tags

From: Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli

Lane

Vector

Fusion tag

BL21

Rosetta 2

20°C PPB

37°C PPB

25°C TBONEX

20°C PPB

37°C PPB

25°C TBONEX

1

pOPINF

6-His-

1

1

1

1

1

1

2

pOPINS3C

6-His-SUMO-

0.7

1.5

2.1

1.3

2.7

1.1

3

pOPINTRX

6-His-Thioredoxin-

1.2

1.7

4.9

1.1

0.7

0.1

4

pOPINMSYB

6-His-MsyB-

0.5

0.8

2.9

1.8

3.6

2.0

5

pOPINJ

6-His-GST-

0.3

1.3

0.4

0.1

0.5

0.1

6

pOPINHALO7

6-His-Halo-

1.5

1.6

1.7

1.6

3.0

2.0

7

pOPINM

6-His-MBP-

0.8

1.4

2.5

0.9

1.1

0.7

8

pOPINTF

6-His-Trigger Factor-

0.5

1.1

1.7

0.3

0.6

0.5

9

pOPINNusA

6-His-NusA-

0.6

1.2

1.1

0.7

0.8

0.1

  1. Empirical scores of soluble fraction yield in small-scale expression relative to 6-His-construct. Highlighted text in bold within the vector name symbolize the fusion tag.