Figure 3

SDS-PAGE analysis of small-scale expression screen. (a) BL21/PPB/20°C; (b) BL21/PPB/37°C; (c) BL21/TBONEX/25°C; (d) Rosetta 2/PPB/20°C; (e) Rosetta 2/PPB/37°C; (f) Rosetta/TBONEX/25°C. Lanes M, 1-9 denote protein marker and Ni-NTA purified soluble hCNTF fusion constructs from expression vectors as listed in Table 1, respectively. The gel was stained with Coomassie Blue dye.