Figure 6

Total yield of target protein production during the bioreactor cultivations. (A) Triplicate cultivations of ZERA-GLA intracellular production showing total ZERA-GLA expressed over time by quantification from anti-ZERA Western blot analysis. Two peaks of ZERA-GLA production are apparent, separated by a decline in the ZERA-GLA level at approximately 30 to 45 hours of the culture. (B) A comparison of CBHI carrier produced as a result of CBHI-GLA production by secretion vs. intracellular ZERA-GLA production over time. By assessing the more stable CBHI carrier by quantitative anti CBHI Western instead of the unstable secreted GLA product it is clear to note that despite the eventual loss of secreted GLA through proteolysis more total GLA was actually produced than with the intracellular ZERA-GLA (quantified by anti ZERA Western).