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Figure 4 | BMC Biotechnology

Figure 4

From: Comparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei

Figure 4

Enzyme activity in relation to recovered yield from insoluble material. A pre-pellet washed batch of PB material was solubilised over a neutral to alkaline pH range and tested for target protein concentration by quantitative Coomassie stain and GLA enzyme activity measurement. A specific activity loss trade-off vs. concentration yield, by solubilisation pH change was established to determine the optimum pH for the procedure.

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BMC Biotechnology

ISSN: 1472-6750

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