manA-df
|
Degenerate primers
|
CGGGTCTGGGGCTTYAAYGAYGT
|
23
|
manA-dr
| |
GTG CCGTAGTAGATGGTRTTNCCRTCRT
|
28
|
manA-3-sp3
|
Amplify gene of the 3’-end by SEFA-PCR
|
ACTTTCCATCTGTACNNNNNNNNNTGTGAG
|
30
|
manA-3-sp2
|
TGACGACCGAGTCTGACGGAAGCTA
|
25
|
manA-3-sp1
|
CTGGGTCAAGGCGACTAGCCAATAT
|
25
|
manA-5-sp3
|
Amplify gene of the 5’-end by SEFA-PCR
|
GTGTTGATGGTGGCGNNNNNNNNNGCAAGA
|
30
|
manA-5-sp2
|
GGAGATGAGATGTACCGCGAAACCA
|
25
|
manA-5-sp1
|
CCATGTGCTTCGGGTCGAGTGATTT
|
25
|
manA-f
|
cDNA cloning primers
|
ATGACATTGGGATTGACTCAGACGA
|
25
|
manA-r
| |
TCAGATCGCAGCGACATGATT
|
21
|
manA-ef
|
Specific expression primers
|
CGGAATTCCAGGTGGCGGAATATGGCCAGTGT
|
32
|
manA-er
| |
GCTCTAGATAGATCGCAGCGACATGATTCGTCACCA
|
36
|
5’AOX
|
Confirmed primers
|
GACTGGTTCCAATTGACAAGC
|
21
|
3’AOX
| |
GCAAATGGCATTCTGACATCC
|
21
|