Table 3 Primers used in this study
Primer name | Primer purpose | Primer sequence | Size (bases) |
|---|---|---|---|
manA-df | Degenerate primers | CGGGTCTGGGGCTTYAAYGAYGT | 23 |
manA-dr | GTG CCGTAGTAGATGGTRTTNCCRTCRT | 28 | |
manA-3-sp3 | Amplify gene of the 3’-end by SEFA-PCR | ACTTTCCATCTGTACNNNNNNNNNTGTGAG | 30 |
manA-3-sp2 | TGACGACCGAGTCTGACGGAAGCTA | 25 | |
manA-3-sp1 | CTGGGTCAAGGCGACTAGCCAATAT | 25 | |
manA-5-sp3 | Amplify gene of the 5’-end by SEFA-PCR | GTGTTGATGGTGGCGNNNNNNNNNGCAAGA | 30 |
manA-5-sp2 | GGAGATGAGATGTACCGCGAAACCA | 25 | |
manA-5-sp1 | CCATGTGCTTCGGGTCGAGTGATTT | 25 | |
manA-f | cDNA cloning primers | ATGACATTGGGATTGACTCAGACGA | 25 |
manA-r | TCAGATCGCAGCGACATGATT | 21 | |
manA-ef | Specific expression primers | CGGAATTCCAGGTGGCGGAATATGGCCAGTGT | 32 |
manA-er | GCTCTAGATAGATCGCAGCGACATGATTCGTCACCA | 36 | |
5’AOX | Confirmed primers | GACTGGTTCCAATTGACAAGC | 21 |
3’AOX | GCAAATGGCATTCTGACATCC | 21 |