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Figure 2 | BMC Biotechnology

Figure 2

From: A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris

Figure 2

SDS-PAGE and zymography analysis of culture supernatants from recombinant Pichia pastoris during 7-day fermentation. SDS-PAGE (A) and zymography (B) of secreted proteins produced by recombinant Pichia pastoris fusing the P. oxalicum GZ-2 endo-β-1, 4-mannosidase gene. Lane CK: Pichia pastoris GS115 with the empty pPICZαA after induction for 4 days; Lane 1-7: culture supernatant after induction for 1, 2, 3, 4, 5, 6, and 7 days; Lane M: protein molecular mass makers. Twenty microliters of culture supernatants was added into each gel lane.

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BMC Biotechnology

ISSN: 1472-6750

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