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Figure 3 | BMC Biotechnology

Figure 3

From: Molecular characterization of two A-type P450s, WsCYP98A and WsCYP76A from Withania somnifera (L.) Dunal: expression analysis and withanolide accumulation in response to exogenous elicitations

Figure 3

Phylogenetic analysis of deduced amino acid sequences of WsCYP98A and WsCYP76A was inferred using the Neighbour-joining method employing MEGA 6.0 software. For WsCYP98A total of 10 sequences and for WsCYP76A, 12 sequences including Withania somnifera were used for analysis. The sequences used were from following plant specieis: Withania somnifera (WsCYP98A: HM036710; WsCYP76A: GU808569), Solanum lycopersicum (ACF17644.1) Capsicum annuum (ACF17644.1) Solanum tuberosum (XP_006347680.1), Ruta graveolens (AEG19446.1), Platycodon grandiflorus (AEM63674.1), Ammi majus (AAT06912.1), Zea mays (ACG25686.1), Triticum aestivum (CAE47490.1), Papaver somniferum (AFK73720.1), Sinopodophyllum hexandrum (AGC29945.1), Solanum melongena (CAA50648.1), Solanumlycopersicum (XP_004249463), Populus trichocarpa (XP_002309107.1), Fragaria vesca (XP_004305369.1), Petunia x hybrida (BAC53892.1), Theobroma cacao (XP_007027589.1), Catharanthus roseus (iridoid oxidase; AHK60833.1), Morus notabilis (EXC33897.1), Rauvolfia serpentina (G-10-H like; AGX93053.1), Amsonia hubrichtii (G-10-H like; AGX93052.1, Citrus sinensis (G-8-H like; XP_006477331.1). Both P450 confined to separated clan and exhibited maximum similarity with their respective members of CYP98 and CYP76 families.

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BMC Biotechnology

ISSN: 1472-6750

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