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Table 1 Kinetic parameters for the digestion of GD 4 K-na by hEPl wt and hEPl scC112S

From: Improvement of an antibody-enzyme coupling yield by enzyme surface supercharging

Enteropeptidase Km[mmol/L] kcat[s−1] kcat/Km[(mmol/L)−1−s−1] Ref.
hEPl wt 0.11 ± 0.01 81.9 ± 9.8 745 This study
hEPl scC112S 0.65 ± 0.09 169.3 ± 24.5 260 This study
Human recombinant light chain 0.16 ± 0.01 115 ± 5 719 [14]