Table 1 Kinetic parameters for the digestion of GD 4 K-na by hEPl wt and hEPl scC112S
From: Improvement of an antibody-enzyme coupling yield by enzyme surface supercharging
Enteropeptidase | Km[mmol/L] | kcat[s−1] | kcat/Km[(mmol/L)−1−s−1] | Ref. |
|---|---|---|---|---|
hEPl wt | 0.11 ± 0.01 | 81.9 ± 9.8 | 745 | This study |
hEPl scC112S | 0.65 ± 0.09 | 169.3 ± 24.5 | 260 | This study |
Human recombinant light chain | 0.16 ± 0.01 | 115 ± 5 | 719 | [14] |