Figure 2From: Improvement of an antibody-enzyme coupling yield by enzyme surface superchargingAssay performances of hEPl wt and hEPl scC112S conjugate solutions. Detection of an immobilized model antigen (HPT-104 antibody [13]) was performed using 2 μg/mL total protein of untreated conjugate solution. After incubation and washing, signal was generated by addition of 50 μmol/L GD4K-na and monitored for 100 minutes. Errors = SD, n = 3.Back to article page