Table 2 Primers used for RT-PCR and real time quantitative PCR
From: AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation
Gene | Primer sequence (5′–3′) | Anneal. T (°C) | Amplicon size | GenBank accession number |
|---|---|---|---|---|
Myostatin | F: 5′-CTGGCCCAGTGGATCTGAATGAG −3′ | 60 | 151 bp | NM_001009428 |
R: 5′- TTCCAGGCGAAGCTTACTGAGGA-3′ | ||||
Follistatin | F: 5′-TGGACCGAGGAGGACGTGAA-3′ | 60 | 160 bp | NM_001257093.1 |
R: 5′-AGACGCAGCGGGGTTTGTTC-3′ | ||||
ActRIIB | F: 5′-TCCCTCACGGATTACCTCAAG-3′ | 58 | 149 bp | NM_174495 |
R: 5′-TCCCTGTGGGCAATAGATGG-3′ | ||||
Myf5 | F: 5′-ACCAGCCCCACCTCAAGTTG-3′ | 60 | 150 bp | NM_174116.1 |
R: 5′-GCAATCCAAGCTGGATAAGGAG-3′ | ||||
MyoD | F: 5′-CGACTCGGACGCTTCCAGT-3′ | 60 | 181 bp | NM_001040478.2 |
R: 5′-GATGCTGGACAGGCAGTCGA-3′ | ||||
P21 | F: 5′-GCAGACCAGCATGACAGATTT-3′ | 58 | 70 bp | NM_001098958 |
R: 5′-GGATTAGGGCTTCCTCTTGGA-3′ | ||||
P57 | F: 5′- CGCCGCACCTTTCCCATG-3′ | 59 | 166 | NM_001142510 |
R: 5′- CCCGCAGTGGCATGTCCA-3′ | ||||
CDK2 | F: 5′-CATGGATGCCTCTGCACTCACTG-3′ | 60 | 102 bp | NM_001142509.1 |
R: 5′-AGGACCCGATGAGAGTGGCAGAA-3′ | ||||
Akt1 | F: 5′-CAGCATCGTGTGGCAGGAC-3′ | 60 | 138 bp | NM_001161857.1 |
R: 5′-TCTTGGTCAGGTGGCGTAA-3′ | ||||
GAPDH | F: 5′-CAAGTTCCACGGCACAGTCA-3′ | 60 | 249 bp | NM_001190390 |
R: 5′-TGGTTCACGCCCATCACAA-3′ |