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Table 2 Primers used for RT-PCR and real time quantitative PCR

From: AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation

Gene Primer sequence (5′–3′) Anneal. T (°C) Amplicon size GenBank accession number
Myostatin F: 5′-CTGGCCCAGTGGATCTGAATGAG −3′ 60 151 bp NM_001009428
R: 5′- TTCCAGGCGAAGCTTACTGAGGA-3′
Follistatin F: 5′-TGGACCGAGGAGGACGTGAA-3′ 60 160 bp NM_001257093.1
R: 5′-AGACGCAGCGGGGTTTGTTC-3′
ActRIIB F: 5′-TCCCTCACGGATTACCTCAAG-3′ 58 149 bp NM_174495
R: 5′-TCCCTGTGGGCAATAGATGG-3′
Myf5 F: 5′-ACCAGCCCCACCTCAAGTTG-3′ 60 150 bp NM_174116.1
R: 5′-GCAATCCAAGCTGGATAAGGAG-3′
MyoD F: 5′-CGACTCGGACGCTTCCAGT-3′ 60 181 bp NM_001040478.2
R: 5′-GATGCTGGACAGGCAGTCGA-3′
P21 F: 5′-GCAGACCAGCATGACAGATTT-3′ 58 70 bp NM_001098958
R: 5′-GGATTAGGGCTTCCTCTTGGA-3′
P57 F: 5′- CGCCGCACCTTTCCCATG-3′ 59 166 NM_001142510
R: 5′- CCCGCAGTGGCATGTCCA-3′
CDK2 F: 5′-CATGGATGCCTCTGCACTCACTG-3′ 60 102 bp NM_001142509.1
R: 5′-AGGACCCGATGAGAGTGGCAGAA-3′
Akt1 F: 5′-CAGCATCGTGTGGCAGGAC-3′ 60 138 bp NM_001161857.1
R: 5′-TCTTGGTCAGGTGGCGTAA-3′
GAPDH F: 5′-CAAGTTCCACGGCACAGTCA-3′ 60 249 bp NM_001190390
R: 5′-TGGTTCACGCCCATCACAA-3′