Real time quantitative PCR results of p21, MyoD, Akt I, CDK2, p57 and Myf5 genes expression in transduced primary myoblasts cultured under proliferating conditions. Total RNA was isolated after 96 h from the OPM transduced with AAV-CFS-FLAG and the non-transduced OPMs (control). Gene expression was determined using real-time qPCR and normalized to GAPDH mRNA. The non-transduced myoblasts and AAV-GFP were selected as the control and negative control, respectively. **, P < 0.01 with non-transduced control.