Figure 4
From: AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation
Real time quantitative PCR results of p21, MyoD, Akt I, CDK2, p57 and Myf5 genes expression in transduced primary myoblasts cultured under proliferating conditions. Total RNA was isolated after 96 h from the OPM transduced with AAV-CFS-FLAG and the non-transduced OPMs (control). Gene expression was determined using real-time qPCR and normalized to GAPDH mRNA. The non-transduced myoblasts and AAV-GFP were selected as the control and negative control, respectively. **, P < 0.01 with non-transduced control.