Figure 4
From: An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody
DNA fingerprint analysis of specific scFv genes of positive clones. scFv inserts were amplified from individual colonies of all positive clones. Amplification was done with Ex-Taq DNA polymearase enzyme using pCANTAB5-R1 and pCANTAB5-S6 primers. The amplified products were then analyzed on agarose gels. A. scFv encoding inserts of DNA of 40 diversified positive clones B. Only five restriction patterns of scFv encoding inserts of DNA were identified against five different clones (scFv K1 - K5). Molecular weights of DNA ladder markers (kbp) were indicated by M on the left.