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Table 1 cDNA yield, dye incorporation and amount of material used for hybridization (n= 6 for each method).

From: A rapid and inexpensive labeling method for microarray gene expression analysis

 

Labeling Yield (avg. ± S.D.)

Quantity hybridized

 

cDNA

(μg)

Dye

(pmol)

Ratio

(pmol dye/μg cDNA)

cDNA (μg)

ds-cDNA

25.8 ± 0.8

463 ± 66

18 ± 3

4.00

Indirect

0.33 ± 0.06

33 ± 5

105 ± 26

0.30

Direct Random

7.7 ± 0.6

85 ± 6

11.1 ± 0.5

5.00

  1. S.D., standard deviation.