Skip to main content

Table 1 cDNA yield, dye incorporation and amount of material used for hybridization (n= 6 for each method).

From: A rapid and inexpensive labeling method for microarray gene expression analysis

  Labeling Yield (avg. ± S.D.) Quantity hybridized
  cDNA
(μg)
Dye
(pmol)
Ratio
(pmol dye/μg cDNA)
cDNA (μg)
ds-cDNA 25.8 ± 0.8 463 ± 66 18 ± 3 4.00
Indirect 0.33 ± 0.06 33 ± 5 105 ± 26 0.30
Direct Random 7.7 ± 0.6 85 ± 6 11.1 ± 0.5 5.00
  1. S.D., standard deviation.
\