Figure 1From: High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle VirusSchematic representation of the p35:Nef and p35:AMCV-P19 constructs. In p35:Nef and p35:AMCV-P19 constructs, nef and amcv-p19 genes are under the control of the CaMV 35S promoter (35S) and the TMV 5'-untranslated leader sequence omega (Ω). nef: cDNA encoding a mutated form of Nef in which the myristoylation consensus sequence is abolished; amcv-p19: cDNA encoding AMCV-P19 gene silencing suppressor; tags: Flag-tag followed by a 6× histidine tag.Back to article page