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Table 3 Plant germplasm.

From: Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations

Line Experiments Transgenic? Generation Parental Lines Purpose
Brassica napus (BN) Both no n/a n/a crop: parental control
Brassica rapa (BR) Both no n/a n/a weed: parental control
GT1 hybrid Both yes: mGFP5er and Bt cry1Ac mixed: BC1/F2 BR and transgenic BN to study event-specific genetic load
GT5 hybrid Both yes: mGFP5er and Bt cry1Ac mixed: BC1/F2 BR and transgenic BN to study event-specific genetic load
GT9 hybrid Both yes: mGFP5er and Bt cry1Ac mixed: BC1/F2 BR and transgenic BN to study event-specific genetic load
TM homozygous [TM(H)] TM only yes: Δgai and ahas T2 BN TM parental control
TM backcross [TM(B)] TM only yes: Δgai and ahas mixed: BC1/F2 BR and transgenic BN To study transgene persistence in a backcrossed population
  1. Summary of line designations, generations and parental lines for the seven plant germplasm types utilized in this study. The transgene nomenclature is as follows: mGFP5er (gene coding for green fluorescent protein); Bt cry1Ac (Bacillus thuringiensis insecticidal toxin gene); Δgai (gibberellic acid insensitive gene); ahas (acetolactate synthase-herbicide resistance gene).
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BMC Biotechnology

ISSN: 1472-6750

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