Table 1 Binding affinity constants (KA) and thermodynamic properties of XGBM-carbohydrate interactions*.
From: Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules
| Module | Carbohydrate | |||||||
|---|---|---|---|---|---|---|---|---|
| xyloglucan | XXXG | XLLG† | galactose | |||||
|
K
A
(103 M-1) |
K
A
(103 M-1) |
K
A
(103 M-1) |
ΔG
(kcal mol-1) |
ΔH
(kcal mol-1) |
TΔS
(kcal mol-1) | n§ | ||
| XG-34 | ≈ 0.1 | ≈ 1 | ≈ 1 | - | - | - | - | not determined |
| XG-34/1-X | ≈ 10 | ≈ 1 | 147 ± 6 | -7.0 ± 0.1 | -15.8 ± 0.1 | -8.8 ± 0.1 | 1.0 ± 0.0 | no binding |
| XG-34/2-VI | ≈ 10 | ≈ 1 | 141 ± 4 | -7.0 ± 0.0 | -14.0 ± 0.1 | -7.0 ± 0.1 | 1.1 ± 0.0 | no binding |