Figure 8

Tamarind seed xyloglucan binding. Staining of tamarind seed tissue sections using fluorescein-labeled XGBM as probes. The probes were used at 200 (A), 40 (B), 20 (C), 4 (D) and 2 (E) μg/ml. F illustrates the lack of staining with a protein-specific module that does not bind plant carbohydrates, G-4 [17] developed from the CBM4-2 scaffold, at 200 μg/ml. All sections were scanned with the same settings resulting in oversaturation of the signal for the best XGBM at the highest concentrations. Bar lines = 200 μm