Figure 7From: Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modulesAsp/Glu112 and binding affinity. AE of XGBM XG-34/2-VI and Asp112 → Glu-mutated XG-34 that both contain glutamate in position 112 demonstrate that they bind stronger to xyloglucan than their aspartate-containing counterparts. None of these modules show binding to xylan that is recognized by the xylan-specific module X-2 [15]. The arrow indicates the direction of migration in the gels.Back to article page