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Figure 7 | BMC Biotechnology

Figure 7

From: Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules

Figure 7

Asp/Glu112 and binding affinity. AE of XGBM XG-34/2-VI and Asp112 → Glu-mutated XG-34 that both contain glutamate in position 112 demonstrate that they bind stronger to xyloglucan than their aspartate-containing counterparts. None of these modules show binding to xylan that is recognized by the xylan-specific module X-2 [15]. The arrow indicates the direction of migration in the gels.

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