Figure 2From: Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modulesCBM selection and evolution scheme. Scheme showing the route of CBM evolution. Targeted mutagenesis of 12 codons encoding the carbohydrate-binding cleft of CBM4-2 resulted in a first library displayed on phage [6] from which the XGBM XG-34 was isolated [14]. Random PCR-based mutagenesis of the gene encoding XG-34 resulted in second generation phage display libraries from which matured variants of XG-34 could be selected by a stringent selection procedure (this investigation).Back to article page