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Table 3 Primer sequences

From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae

Molecular Assay Primers

An. gambiae species ID/rDNA PCR

(5' to 3') Fragment size (bp)

IMP-UN*

GCTGCGAGTTGTAGAGATGCG

F

 

QD-3T*

GCATGTCCACCAACGTAAAtCC

R

An. quadriannulatus 637

ME-3T*

CAACCCACTCCCTTGACGaTG

R

An. melas and merus 529

GA-3T*

GCTTACTGGTTTGGTCGGCAtGT

R

An. gambiae 464

AR-3T*

GTGTTAAGTGTCCTTCTCCgTC

R

An. arabiensis 388

IMP-M1

TAGCCAGCTCTTGTCCACTAGTtTT

R

Mopti 333

IMP-S1

CCAGACCAAGATGGTTCGcTG

R

Savanna 221

An. gambiae white gene PCR

UFOR

ATTATCTGATGAAGCTTGGAGTCTTTT

F

 

UREV

ATGAAAATAAGGAGCTTCCTGGCAT

R

positive control 478

MOPR

CTGTTGTCTTACAGTAGGGTTAtGT

R

An. gambiae MOPTI 413

MOP2R

AACGTACGACGTATGATCTAACtGA

R

An. gambiae MOPTI/ZAN/U 350

MALR

CTCATATTCAAGGATGAACACAAtAC

R

An. gambiae MALI 292

PIMPR

TCAATGACATGACGTTATAATCTGTCtTT

R

An. gambiae PIMPERENA 116

  1. Primers indicated with (*) obtained from Wilkins et al [17]. F and R indicate forward and reverse orientation. Lower case indicates site of intentional mismatch. Bold nucleotides show the site of the SNP.