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Table 1 Authentication table example for An. gambiae

From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae

 

Morphological Assay

Insecticide Assay

Molecular Assay

Test

mel

eye

c+/cc

Perm

Diel

DDT

Prop

2La:TEP1

GA/rDNA

white

Marker

1

2

3

4

5

6

7

8

9

10

4ARR

no

pink

        

M2

no

white

        

RMOSP

no

mosaic

        

MALI

yes

wild

c+

no

no

no

no

2La/2La:r

GA/M

Mali

RSP-ST

yes

wild

c+

yes

no

no

no

2L+a/2L+a:s

  

AKRON

yes

wild

c+

no

no

no

yes

   

SUA

yes

wild

c+

no

no

no

no

2La/2La:s

  

PIMPER

yes

wild

c+

no

no

no

no

2L+a/2La:r/s

 

Pimper

MOPTI

yes

wild

c+/cc

no

no

no

no

2La/2La:r

GA/M

Mopti

ZAN/U

yes

wild

c+/cc

no

no

yes

   

Zan/u

G3

yes

wild

c+/cc

no

no

no

no

2L+a/2La:s

GA/MS

 

IN22C+

y/n*

wild

c+/cc

no

yes

     

RSP

yes

wild

c+/cc

yes

      

KISUMU

yes

wild

c+/cc

no

no

no

no

2L+a/2La:s

GA/S

 

ASEMBO

yes

wild

cc

no

no

no

no

2L+a/2L+a:s

  

L3-5

yes

wild

cc

no

no

no

no

2L+a/2L+a:r

  
  1. Authentication table for MR4 An. gambiae s.s. colonies detailing morphological and molecular characteristics useful for differentiation. Order of tests from left to right: mel: Homochromy (larval cuticular darkening), eye color (pupal or adult), c+/cc (collarless trait), permethrin resistance, dieldrin resistance, DDT resistance, propoxur resistance, PCR: 2La and TEP1, PCR: An. gambiae species identification and rDNA, PCR: white gene. * IN22C+ population is polymorphic for this trait.