| |
Morphological Assay
|
Insecticide Assay
|
Molecular Assay
|
|---|
|
Test
|
mel
|
eye
|
c+/cc
|
Perm
|
Diel
|
DDT
|
Prop
|
2La:TEP1
|
GA/rDNA
|
white
|
|---|
|
Marker
|
1
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
9
|
10
|
|
4ARR
|
no
|
pink
| | | | | | | | |
|
M2
|
no
|
white
| | | | | | | | |
|
RMOSP
|
no
|
mosaic
| | | | | | | | |
|
MALI
|
yes
|
wild
|
c+
|
no
|
no
|
no
|
no
|
2La/2La:r
|
GA/M
|
Mali
|
|
RSP-ST
|
yes
|
wild
|
c+
|
yes
|
no
|
no
|
no
|
2L+a/2L+a:s
| | |
|
AKRON
|
yes
|
wild
|
c+
|
no
|
no
|
no
|
yes
| | | |
|
SUA
|
yes
|
wild
|
c+
|
no
|
no
|
no
|
no
|
2La/2La:s
| | |
|
PIMPER
|
yes
|
wild
|
c+
|
no
|
no
|
no
|
no
|
2L+a/2La:r/s
| |
Pimper
|
|
MOPTI
|
yes
|
wild
|
c+/cc
|
no
|
no
|
no
|
no
|
2La/2La:r
|
GA/M
|
Mopti
|
|
ZAN/U
|
yes
|
wild
|
c+/cc
|
no
|
no
|
yes
| | | |
Zan/u
|
|
G3
|
yes
|
wild
|
c+/cc
|
no
|
no
|
no
|
no
|
2L+a/2La:s
|
GA/MS
| |
|
IN22C+
|
y/n*
|
wild
|
c+/cc
|
no
|
yes
| | | | | |
|
RSP
|
yes
|
wild
|
c+/cc
|
yes
| | | | | | |
|
KISUMU
|
yes
|
wild
|
c+/cc
|
no
|
no
|
no
|
no
|
2L+a/2La:s
|
GA/S
| |
|
ASEMBO
|
yes
|
wild
|
cc
|
no
|
no
|
no
|
no
|
2L+a/2L+a:s
| | |
|
L3-5
|
yes
|
wild
|
cc
|
no
|
no
|
no
|
no
|
2L+a/2L+a:r
| | |
- Authentication table for MR4 An. gambiae s.s. colonies detailing morphological and molecular characteristics useful for differentiation. Order of tests from left to right: mel: Homochromy (larval cuticular darkening), eye color (pupal or adult), c+/cc (collarless trait), permethrin resistance, dieldrin resistance, DDT resistance, propoxur resistance, PCR: 2La and TEP1, PCR: An. gambiae species identification and rDNA, PCR: white gene. * IN22C+ population is polymorphic for this trait.
