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Table 4 Effect of co-substrate concentration on the bioreduction

From: Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells

2-Propanol concentration (mM) V o
(μmol/min gcwm)
Yielda
(%)
e.e.b
(%)
0 0.95 25.6 >99
34 1.57 72.9 >99
66 1.99 85.1 >99
98 2.30 92.8 >99
130 2.46 97.7 >99
162 2.22 90.5 >99
194 1.88 80.3 >99
258 1.07 50.4 >99
  1. Reaction conditions: 24 mM TMSB, C4MIM·PF6/TEA-HCl buffer (100 mM, pH 5.5) volume ratio of 1/4, various concentrations of 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min.
  2. aMaximum yield.
  3. bProduct e.e.