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Table 4 Effect of co-substrate concentration on the bioreduction

From: Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells

2-Propanol concentration (mM)

V o

(μmol/min gcwm)

Yielda

(%)

e.e.b

(%)

0

0.95

25.6

>99

34

1.57

72.9

>99

66

1.99

85.1

>99

98

2.30

92.8

>99

130

2.46

97.7

>99

162

2.22

90.5

>99

194

1.88

80.3

>99

258

1.07

50.4

>99

  1. Reaction conditions: 24 mM TMSB, C4MIM·PF6/TEA-HCl buffer (100 mM, pH 5.5) volume ratio of 1/4, various concentrations of 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min.
  2. aMaximum yield.
  3. bProduct e.e.