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Table 3 Effect of TMSBOL addition on the bioreduction conducted in C4 MIM·PF6/buffer biphasic system

From: Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells

Addition of

TMSBOL (mM)

V o

(μmol/min gcwm)

Yielda

(%)

e.e.b

(%)

0

2.18

89.6

>99

3

1.88

84.5

>99

6

1.67

78.1

>99

12

1.34

68.8

>99

24

0.75

33.7

>99

  1. Reaction conditions: 24 mM TMSB, C4MIM·PF6/TEA-HCl buffer (100 mM, pH 5.0) volume ratio of 1/4, 98 mM 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min.
  2. aMaximum yield.
  3. bProduct e.e.