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Table 1 Effect of various water-immiscible ILs on the bioreduction

From: Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells

Medium Viscosity of IL
(cP, 30°C)
V o a
(μmol/min gcwm)
Yieldb
(%)
e.e.c
(%)
Aqueous buffer - 1.18d 33.6d >99d
C4MIM·PF6/buffer 173 1.56 85.0 >99
C5MIM·PF6/buffer 240 1.41 81.2 >99
C6MIM·PF6/buffer 312 1.30 77.4 >99
C7MIM·PF6/buffer 355 1.23 72.3 >99
iC4MIM·PF6/buffer 238 1.04 63.1 >99
C2MIM·Tf2N/buffer 25 1.21 71.6 >99
C4MIM·Tf2N/buffer 44 1.08 64.7 >99
  1. Reaction conditions: 12 mM TMSB, IL/TEA-HCl buffer (100 mM, pH 5.0) volume ratio of 1/2, 98 mM 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min.
  2. aInitial reaction rate (Vo) is defined as the initial rate of the product formation in the total reaction system, expressed as the specific activity in μmol product per min per gram of cell wet mass (cwm) unless specified otherwise.
  3. bMaximum yield.
  4. cProduct e.e.
  5. dData taken from Zhang et al [10].