Figure 5
Effect of substrate concentration on the biocatalytic reduction of TMSB with immobilized Candida parapsilosis CCTCC M203011 cells in the C 4 MIM·PF 6 -based system (A) and in the aqueous system (B). Reaction conditions: (A): various concentrations of TMSB, C4MIM·PF6/TEA-HCl buffer (100 mM, pH 5.0) volume ratio of 1/4 (0.5 mL/2.0 mL), 98 mM 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min; (B): various concentrations of TMSB, TEA-HCl buffer (4 mL, 100 mM, pH 5.0), 98 mM 2-propanol, 0.15 g/mL cell-loaded alginate beads, 30°C, 180 r/min. Symbols: (○) product e.e.; (□) maximum yield; (Δ) initial reaction rate.