Table 1 Genes analyzed in RT-PCR experiments, primer sequences and annealing temperature.
From: Isolation of osteogenic progenitors from human amniotic fluid using a single step culture protocol
Gene | Gene symbol | Primer Sequences | Annealing temperature | Size (bp) |
|---|---|---|---|---|
Stromal cell-derived factor-1 | SDF1 | F – gacccgcgctcgtccgcc R – cgggtcaatgcacacacttgtcta | 57° | 262 |
Chemokine (C-X-C motif) receptor 4 | CXCR4 | F – agctgttggctgaaaaggtgg R – gcgcttctggtggcccttgga | 60° | 260 |
Octamer-binding transcription factor 4 | Oct-4 | F – cgt gaa gct gga gaa gga gaa gct g R – caa ggg ccg cag ctc aca cat gtt c | 60° | 245 |
Stem cell factor | SCF | F – cca ttg atg cct tca agg ac R – ctt cca gta taa ggc tcc aa | 62° | 275 |
GATA binding protein 4 | GATA-4 | F – ttc ctc ttc cct cct caa at R – tca gcg tgt aaa ggc atc tg | 60° | 194 |
Vimentin | Vim | F – tca gcg tgt aaa ggc atc tg R – cct tcg tga ata cca cg acct gc | 56° | 321 |
Fibroblast growth factor 5 | FGF-5 | F – gct gtg tct cag ggg att gta gga ata R – tat cca aag cga aac ttg agt ctg ta | 62° | 434 |
Paired box 6 | Pax-6 | F – aga ttc aga tga ggc tca aa R – aat tgg ttg gta gac act gg | 60° | 313 |
Neural cell adhesion molecule | NCAM | F – gag ggg gaa gat gcc gtg atg tg R – ata ttc tgc ctg gcc cgg atg gta g | 63° | 269 |
Bone morphogenetic protein 2 | BMP-2 | F – ttg cgg ctg ctc agc atg tt R – ttg cga gaa cag atg caa gat g | 62° | 315 |
Alpha-fetoprotein | AFP | F – gtg ctg cac ttc ttc ata tgc R – tga cag cct caa gtt gtt cc | 60° | 218 |
Type I collagen | COL1 | F – ttcctttgcattcatctctca R – caagtggaccaagcttcctt | 58° | 149 |
Osteonectin | ONC | F – gtctcactggctgtgttgga R – aagacttgccatgtgggttc | 60° | 215 |
Osteopontin | OPN | F – aggaggaggcagagcaca R – ctggtatggcacaggtgatg | 60° | 152 |
Osteocalcin | OCN | F – catgagagccctcaca R – agagcgacaccctagac | 58° | 315 |
Osteoprotegerin | OPG | F – tgctgttcctacaaagttttacg R – ctttgagtgctttagtgcgtg | 60° | 433 |
Bone sialoprotein | BSP | F – ctatggaaggacgccacgcct R – catagccatcgtagccttgtcc | 62° | 578 |
Runt-related transcription factor 2 | Runx2 | F – gacagaagcttgatgactctaaacc R – tctgtaatctgactctgtccttgt | 60° | 169 |
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | F – ccatggagaaggctggg R – caaagttgtcatggatgacc | 60° | 194 |