Figure 2
From: Isolation of osteogenic progenitors from human amniotic fluid using a single step culture protocol
a) Fibroblast-like cells (AFMSCs) obtained after 7 days of amniotic fluid culture (Protocol 1); b) Confluence of AFMSCs after 22 days of amniotic fluid culture (Protocol 1); c) over confluent osteoblastic cells after 20 days of amniotic fluid culture in osteogenic medium (Protocol 2); d) nodules of calcium mineralization, osteoblastic cells (Protocol 2); e) Alizarin Red Staining of osteoblastic cells obtained after 22 days of amniotic fluid culture. Red spots indicate the presence of calcium mineralization; f) Alizarin Red Staining of osteoblastic cells after 30 days of amniotic fluid culture. Note the increase in the number and size of aggregates of calcium mineralization.