Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes

Table 3 Hepatic functions of different hepatic cell lines and primary mature human hepatocytes (Mat Hep)

	Hepatic function (n = 9)			mRNA levels (n = 3) Mature hepatic marker				mRNA levels (n = 3) Immature hepatic marker
Cell	Albumin production (ng/h/mg protein)	Urea production (nmol/h/mg protein)	Galactose elimination (μmol/h/mg protein)	Albumin	Transferrin	AAT	HNF4	GSTπ	AFP
Mat Hep	37.7 ± 7.7	91.5 ± 33.7	0.58 ± 0.44	130	245	121	141	535	ND
cBAL08	0.3 ± 0.4	4.0 ± 1.6	ND	0.02 ± 0.01	0.20 ± 0.14	0.32 ± 0.14	ND	1000 ± 565	78 ± 61
cBAL111 day 2	Undetectable	Undetectable	0.04 ± 0.02	0.02 ± 0.01	0.11 ± 0.05	0.02 ± 0.01	1.3 ± 1.3	1944 ± 1010	122 ± 11
cBAL111 day 15	0.7 ± 0.8	8.0 ± 6.6	0.11 ± 0.03*	0.02 ± 0.01	0.20 ± 0.06	0.05 ± 0.01	17.2 ± 5.6*	1374 ± 671	114 ± 10
HepG2	2.8 ± 0.3	4.7 ± 0.2	ND	63 ± 6	896 ± 110	199 ± 56	ND	Undetectable	93353 ± 13228
NKNT-3 reverted	Undetectable	4.9 ± 9.4	ND	0.24 ± 0.22	0.16 ± 0.12	0.92 ± 1.52	ND	952 ± 1164	117 ± 166
NKNT-3 unreverted	Undetectable	2.9 ± 5.2	ND	0.42 ± 0.66	0.20 ± 0.13	0.26 ± 0.34	ND	4427 ± 5366	65 ± 79

MessengerRNA levels are expressed as a percentage of the mean mRNA levels of the two liver samples. ND = not determined
* cBAL111 day 2 versus cBAL111 day 15; P < 0.05

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