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Table 3 Hepatic functions of different hepatic cell lines and primary mature human hepatocytes (Mat Hep)

From: Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes

  Hepatic function (n = 9) mRNA levels (n = 3)
Mature hepatic marker
mRNA levels (n = 3)
Immature hepatic marker
Cell Albumin production
(ng/h/mg protein)
Urea production
(nmol/h/mg protein)
Galactose elimination
(μmol/h/mg protein)
Albumin Transferrin AAT HNF4 GSTπ AFP
Mat Hep 37.7 ± 7.7 91.5 ± 33.7 0.58 ± 0.44 130 245 121 141 535 ND
cBAL08 0.3 ± 0.4 4.0 ± 1.6 ND 0.02 ± 0.01 0.20 ± 0.14 0.32 ± 0.14 ND 1000 ± 565 78 ± 61
cBAL111
day 2
Undetectable Undetectable 0.04 ± 0.02 0.02 ± 0.01 0.11 ± 0.05 0.02 ± 0.01 1.3 ± 1.3 1944 ± 1010 122 ± 11
cBAL111
day 15
0.7 ± 0.8 8.0 ± 6.6 0.11 ± 0.03* 0.02 ± 0.01 0.20 ± 0.06 0.05 ± 0.01 17.2 ± 5.6* 1374 ± 671 114 ± 10
HepG2 2.8 ± 0.3 4.7 ± 0.2 ND 63 ± 6 896 ± 110 199 ± 56 ND Undetectable 93353 ± 13228
NKNT-3 reverted Undetectable 4.9 ± 9.4 ND 0.24 ± 0.22 0.16 ± 0.12 0.92 ± 1.52 ND 952 ± 1164 117 ± 166
NKNT-3 unreverted Undetectable 2.9 ± 5.2 ND 0.42 ± 0.66 0.20 ± 0.13 0.26 ± 0.34 ND 4427 ± 5366 65 ± 79
  1. MessengerRNA levels are expressed as a percentage of the mean mRNA levels of the two liver samples. ND = not determined
  2. * cBAL111 day 2 versus cBAL111 day 15; P < 0.05