Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes

Table 1 Primers and conditions used in RT-PCR analysis

Gene	Sense primer 5' → 3'	Antisense primer 5' → 3'	Application	Size amplicon (bp)	PCR conditions
					Dilution template	Annealing temp. (°C)
*18S rRNA*	NA	CGAACCTCCGACTTTCGTTT	RT		NA
*AAT*	NA	GGGGGATAGACATGGGTATGG	RT		NA
*AFP*	NA	CGTTTTGTCTTCTCTTCCCC	RT		NA
*Albumin*	NA	ACTTCCAGAGCTGAAAAGCATGGTC	RT		NA
*GSTπ*	NA	AGCAGGTCCAGCAGGTTG	RT		NA
*HNF4α*	NA	CACTCCAACCCCGCCCCTC	RT		NA
*hTERT*	NA	CAGAGCAGCGTGGAGAGGATG	RT		NA
*Transferrin*	NA	CCAGACCACACTTGCCCGCTATG	RT		NA
*18S rRNA*	TTCGGAACTGAGGCCATGAT	CGAACCTCCGACTTTCGTTT	PCR	151	1000×	68 → 63
*AAT*	ACAGAAGGTCTGCCAGCTTC	GATGGTCAGCACAGCCTTAT	PCR	181	-	68 → 63
*AFP*	TKCCAACAGGAGGCYATGC	CCCAAAGCAKCACGAGTTTT	PCR	306	-	62 → 55
*Albumin*	TGAGCAGCTTGGAGAGTACA	GTTCAGGACCACGGATAGAT	PCR	189	-	68 → 63
*GSTπ*	GCCAGAGCTGGAAGGAGG	TTCTGGGACAGCAGGGTC	PCR	333	10×	70 → 63
*HNF4α*	CCGGGTGTCCATACGCATCCT	CAGGTTGTCAATCTTGGCC	PCR	321	-	68 → 63
*hTERT*	CGTACTGCGTGCGTCGGTAT	GGTGGCACATGAAGCGTAGG	PCR	233	-	68 → 63
*Transferrin*	GAAGGACCTGCTGTTTAAGG	CTCCATCCAAGCTCATGGC	PCR	310	-	68 → 63

Sequences of the primers used in Reverse Transcriptase (RT) reaction or during real-time PCR reaction (PCR) and the conditions used in the real-time PCR reactions. NA = not applicable.

ISSN: 1472-6750