Gene
|
Sense primer 5' → 3'
|
Antisense primer 5' → 3'
|
Application
|
Size amplicon (bp)
|
PCR conditions
|
---|
| | | | |
Dilution template
|
Annealing temp. (°C)
|
---|
18S rRNA
|
NA
|
CGAACCTCCGACTTTCGTTT
|
RT
| |
NA
| |
AAT
|
NA
|
GGGGGATAGACATGGGTATGG
|
RT
| |
NA
| |
AFP
|
NA
|
CGTTTTGTCTTCTCTTCCCC
|
RT
| |
NA
| |
Albumin
|
NA
|
ACTTCCAGAGCTGAAAAGCATGGTC
|
RT
| |
NA
| |
GSTπ
|
NA
|
AGCAGGTCCAGCAGGTTG
|
RT
| |
NA
| |
HNF4α
|
NA
|
CACTCCAACCCCGCCCCTC
|
RT
| |
NA
| |
hTERT
|
NA
|
CAGAGCAGCGTGGAGAGGATG
|
RT
| |
NA
| |
Transferrin
|
NA
|
CCAGACCACACTTGCCCGCTATG
|
RT
| |
NA
| |
18S rRNA
|
TTCGGAACTGAGGCCATGAT
|
CGAACCTCCGACTTTCGTTT
|
PCR
|
151
|
1000×
|
68 → 63
|
AAT
|
ACAGAAGGTCTGCCAGCTTC
|
GATGGTCAGCACAGCCTTAT
|
PCR
|
181
|
-
|
68 → 63
|
AFP
|
TKCCAACAGGAGGCYATGC
|
CCCAAAGCAKCACGAGTTTT
|
PCR
|
306
|
-
|
62 → 55
|
Albumin
|
TGAGCAGCTTGGAGAGTACA
|
GTTCAGGACCACGGATAGAT
|
PCR
|
189
|
-
|
68 → 63
|
GSTπ
|
GCCAGAGCTGGAAGGAGG
|
TTCTGGGACAGCAGGGTC
|
PCR
|
333
|
10×
|
70 → 63
|
HNF4α
|
CCGGGTGTCCATACGCATCCT
|
CAGGTTGTCAATCTTGGCC
|
PCR
|
321
|
-
|
68 → 63
|
hTERT
|
CGTACTGCGTGCGTCGGTAT
|
GGTGGCACATGAAGCGTAGG
|
PCR
|
233
|
-
|
68 → 63
|
Transferrin
|
GAAGGACCTGCTGTTTAAGG
|
CTCCATCCAAGCTCATGGC
|
PCR
|
310
|
-
|
68 → 63
|
- Sequences of the primers used in Reverse Transcriptase (RT) reaction or during real-time PCR reaction (PCR) and the conditions used in the real-time PCR reactions. NA = not applicable.