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Figure 2 | BMC Biotechnology

Figure 2

From: Regulated expression of a transgene introduced on an oriP/EBNA-1 PAC shuttle vector into human cells

Figure 2

Stable maintenance of pPAC7 in SK-BR-3 cells. Schematic presentation of the CDH3 and CDH1 genes with the positions on human chromosome 16q22.1 shown in Mb. The 132 kb insert cloned in pPAC7-CDH3, and the positions of the PCR primers used are indicated. DNA isolated from the 293T, SK-BR-3 (S), SK-BR-3/pPAC7 (S-pPAC7) and SK-BR-3/pPAC7-CDH3 (S-CDH3) cell lines was amplified by PCR using the primers indicated [see Additional file 2]. In these experiments the control primers in the NAE1 gene gave a PCR product both from SK-BR-3 and 293T, while no products were detected from SK-BR-3 using the 3 primer pairs from the CDH1-CDH3-region. Selected fragments in the GeneRulerâ„¢ 100 bp DNA Ladder (Fermentas, MD, USA) are indicated.

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