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Figure 8 | BMC Biotechnology

Figure 8

From: Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair

Figure 8

Structure of proviral 5' LTRs after reverse transcription and integration. The predicted structures of the 5' LTRs, both SIN and repaired, for the pHIV-1SDmSneo, pRK1neo and pRK1Δatt340/184neo vectors, and the corresponding size of the expected PCR product from analysis of genomic DNA with the primers int19 and Nar1R are shown. (A) pHIV-1SDmSneo SIN LTR; (B) pRK1neo/pRK1Δatt340/184neo SIN LTR; (C) pRK1Δatt340/184neo repaired LTR; (D) pHIV-1SDmSneo/pRK1neo repaired LTR. See Methods for details and Table 2 for results.

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BMC Biotechnology

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