Figure 2From: Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repairEffect of 3'U3 alterations on virus titre. (A) Titres of vectors containing either the SV40 or bGH polyadenylation sites, and either a 12 or 19 bp att sequence were compared using 0.125 ug vector per well (n = 3). bGHatt12, pHIV-1SDm2SEbGHatt12; bGHatt19, pHIV-1SDm2SEbGHatt19; SVatt12, pHIV-1SDm2SESVatt12; SVatt19, pHIV-1SDm2SESVatt19. * = P < 0.05 ANOVA on Ranks. All results are presented as Mean ± SD. (B) Schematic of 3' LTR structures. In both cases R is truncated at the 3' end, att is 12 or 19 bp in length. SV40, SV40 early polyadenylation signal; bGH, bovine growth hormone gene polyadenylation signal (see Results for details).Back to article page