Pseudotype | HeLa | HepG2 | RAW | Specificity HepG2 vs HeLa | Specificity RAW vs HeLa | Specificity HepG2 vs RAW |
|---|
wtGP64 | 4.2 × 105 ± 3.1 × 105 | 2.6 × 106 ± 1.6 × 106 | 4.0 × 105 ± 3.2 × 105 | 6.1 | 0.15 | 6.5 |
Sendai-GP64 | UDa | 2.7 × 104 ± 1.3 × 104 | UDa | > 27 | NDb | ND |
Sendai-GP64/wtGP64 | 1.8 × 104 ± 1.1 × 104 | 5.1 × 105 ± 2.7 × 105 | 0.3 × 105 ± 0.2 × 105 | 28 | 0.06 | 17 |
Sendai-spGP64 | UDa | 1.2 × 104 ± 0.4 × 104 | UDa | > 12 | NDb | ND |
Sendai-spGP64/wtGP64 | 2.2.104 ± 0.5 × 104 | 4.1 × 105 ± 1.2 × 105 | 2.3 × 105 ± 1.3×105 | 18 | 0.56 | 1.8 |
- Lentiviral vectors were pseudotyped with: wild type GP64, Sendai-GP64 fusion protein, or generated with a mix of 29:1 of fusion- to wild type GP64 expression vector,, were used to transduce HeLa cervical carcinoma cells, HepG2 hepatoma cells and RAW macrophages.
- Viral titers were determined using unconcentrated lentiviral vectors from at least two different virus preparations and are expressed as the amount of transducing units per ml. Specificity was calculated as the ratio of titers on HepG2 and HeLa cells and as the ratio of titers on RAW macrophages and HeLa cells.
- a Undetectable with detection limit of viral titers set to 1 × 103 TU/ml with flow cytometry. No GFP expressing cells were observed using a fluorescent microscope.
- bNot determined.
