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Figure 5 | BMC Biotechnology

Figure 5

From: Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californicaGP64 and Sendai virus F2 domain

Figure 5

PCR analysis of integrated vector genomes. Genomic liver DNA from control mice and mice injected with different GP64 pseudotypes was isolated and PCR specific for integrated lentiviral vectors was performed. Lanes 1 and 2 wtGP64, 3 and 4 Sendai-GP64/wtGP64, 5 and 6 Sendai-spGP64/wtGP64, 7 control liver DNA, 8 water control. The strongest signals are seen in wild type GP64 transduced mice, confirming that these mice have more total GFP positive cells in the liver.

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BMC Biotechnology

ISSN: 1472-6750

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