Immunofluorescent staining of liver macrophages. Liver sections were stained with an antibody against the F4/80 Kupffer cell marker. Kupffer cells are shown in red, GFP positive cell are shown in green. (A-C) Sections from a wtGP64 transduced mouse (A) GFP (B) Kupffer cells (C) composite. (D-F) Sections from a Sendai-GP64/wtGP64 transduced mouse (D) GFP (E) Kupffer cells (F) composite. (G-I) Sections from a Sendai-spGP64/wtGP64 transduced mouse (G) GFP (H) Kupffer cells (I) composite. Images are at 400× magnification. Yellow arrows indicate GFP positive Kupffer cells, grey arrows indicate GFP negative Kuppfer cells, and white stars denote GFP positive hepatocytes. In wild type GP64 and to a lesser extent Sendai-spGP64/wtGP64 injected mice many GFP positive Kupffer cells are observed. In Sendai-GP64/wtGP64 injected mice the level of Kupffer cell tranduction is much lower.