Table 1 PCR primers and PCR conditions.
| Gene | Primers | Tm (°C) | No. of cycles | Product size (bp) | Reference |
|---|---|---|---|---|---|
| CD44v6 | 5'-GACACATATTGCTTCAATGCTTCAGC-3' | 60 | 35 | 348 | [14] |
| 5'-TACTAGGAGTTGCCTGGATGGTAG-3' | |||||
| VEGF-C | 5'-AGACTCAATGCATGCCACG-3' | 57 | 35 | 435 | [15] |
| 5'-TTGAGTCATCTCCAGCATCC-3' | |||||
| MMP-9 | 5'-GTGCTGGGCTGCTGCTTTGCTG-3' | 60 | 35 | 303 | [15] |
| 5'-GTCGCCCTCAAAGGTTTGGAAT-3' | |||||
| β-actin | 5'-CGTCTGGACCTGGCTGGCCGGGACC-3' | 56 | 28 | 600 | [16] |
| 5'-CATGAAGCATTTGCGGTGGACGATG-3' |