Characterization of NT2 cells expanded as 3D-aggregates. Immunofluorescence images of cells from the inoculum (day 0) and collected from the bioreactor culture (day 3 and day 6). Immunolabeling of Oct-4, TRA-1-60, SSEA-4 (green) and nestin (red). Nuclei are labeled with DAPI (blue) (A). Immunofluorescence images of differentiated cultures derived from the inoculum (day 0) and from the bioreactor culture (day 3 and day 6). Neurons labeled with βIII-Tub and MAP2 (green) (B). Nuclei were stained with DAPI (blue). Scale bars: 100 μm.