Identification of the Tol2 construct genomic insertion sites on the T2EC clone #1. A: Splinkerette PCR products on 1.5% agar gel. 1: Fermentas DNA Ladder Mix; 2: genomic DNA from non-transfected T2EC; 3: pT2.CMV-hKO plasmid; 4: genomic DNA from T2EC clone #1, cotransfected with the pT2.CMV-hKO plasmid and the transposase plasmid, pCAGGS-T2TP (molecular ratio: 5/1). B: Alignment of the splinkerette PCR product with the Gallus gallus genome. The sequence corresponding to the Tol2 construct is shown in bold. C: Localization of the Tol2 construct insertion site into chromosome 4 of the Gallus gallus genome.